Potato Virus Y-Resistance in the Progeny of Haploid Mutants Obtained by the Culture of Nicotiana Tabacum L. Anthers Exposed to Ion Beams

Abstract

For chromosome duplication of Potato virus Y (PVY)-resistant haploid mutants obtained by the combination treatment of tobacco anther culture and ion beam exposure, pith tissue culture was performed. Thirty-five plants were obtained by pith tissue culture and subsequent acclimatization. Their chromosome number ranged from 22 to 96, and 16 of them had 48 chromosomes. These plants produced fertile seeds by self-pollination. When plants derived from self-pollinated seeds were inoculated with PVY, resistant and susceptible plants were observed. Of the plants derived from the most resistant mutant in the haploid generation, about 50% was resistant to PVY.