Analysis of Stem Nodule from Sesbania rostrata Using a New Visualization Technique of Three-Dimensional Reconstruction

Abstract

A three-dimensional (3-D) analysis technique was developed to visualize the whole nodule and Nitrogen fixation (N₂-fix) zone in a stem nodule from Sesbania rostrata infected with symbiotic rhizobium, Azorhizobium caulinodans ORS571. The reconstructed N₂-fix zone of the stem nodule was analyzed by arranging the ratio of opacity, by cutting at vertical and level direction, and by extraction in a personal computer. The N₂-fix zone was a ring shape, surrounding the vascular bundle. This is the first report that shows whole distribution of N₂-fix zone in S. rostrata stem nodule.