Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Multiple shoot formation and plant regeneration of a commercially-useful tropical plant, Buchanania lanzan (Spreng)
Sarika ShendeMahendra Rai
Author information

2005 Volume 22 Issue 1 Pages 59-61


Buchanania lanzan (Spreng) is an endemic but vulnerable plant from the tropical region of India. A tissue culture technique for the rapid clonal multiplication of B. lanzan was developed. The decoated seeds were cultured on MS medium enriched with various concentrations of auxins and cytokinins alone or in combination. Combinations of Benzyl amino purine (BAP) and napthalene acetic acid (NAA) were found to be superior to BAP and Indole butyric acid (IBA). Murashige-Skoog (MS) medium supplemented with 22.2 µM of BAP and 5.37 µM of NAA promoted formation of the maximum number of shoots. Furthermore, MS medium containing 23.3 µM kinetin induced profuse rooting of the initiated shoots. We propose here that multiple shoot formation is one of the effective techniques for rapid clonal multiplication.

Information related to the author
© 2005 by Japanese Society for Plant Biotechnology
Previous article Next article