Plant regeneration from leaf explants of auricula cultivars (Primula×pubescens Jacq.)

Abstract

Adventitious shoots were regenerated from leaf explants of 4 cultivars of auricula (Primula×pubescens Jacq.) on media supplemented with various cytokinins combined with NAA. One to four adventitious shoots per leaf explant were regenerated. The highest frequency of adventitious shoot formation was 46.6% for ‘Borders Mixed’ on the medium containing 0.2 mg l⁻¹ TDZ and 2 mg l⁻¹ NAA , 10% for ‘Border Stripes’ on the medium containing 0.2 mg l⁻¹ TDZ and 2 mg l⁻¹ NAA, 20% for ‘Alpines Mixed’ on the medium containing 0.02 mg l⁻¹ TDZ and 2 mg l⁻¹ NAA, 13.3% for ‘Field House Mixed’ on the medium containing 0.02 mg l⁻¹ TDZ and 2 mg l⁻¹ NAA. A higher concentration of NAA (2 mg l⁻¹) combined with TDZ in the medium may stimulate adventitious shoot formation from leaf explants in auricula cultivars. Zeatin was less effective for adventitious shoot regeneration from leaf explants. Regenerated shoot easily rooted on plant growth regulator-free LS medium. After acclimatization in a growth chamber, some of the regenerated plants flowered.