Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Invited Reviews
Gateway vectors for plant transformation
Tsuyoshi NakagawaSumie IshiguroTetsuya Kimura
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JOURNAL FREE ACCESS

2009 Volume 26 Issue 3 Pages 275-284

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Abstract

The availability of complete plant genome sequences has opened a new era of plant molecular research using approaches such as comprehensive expression analysis, ectopic expression and gene knockdown. For such purposes, new tools realizing easy and rapid construction of recombinant plasmids are required. Here, we describe Gateway cloning compatible binary vectors for post-genomic research in plant genetic engineering. The vectors comprise a variety of reporters, epitope tags and selective markers that should make them useful for construction of plasmids for Agrobacterium-mediated transformation of plants. We also describe vectors for promoter swapping using Gateway cloning technology.

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© 2009 by Japanese Society for Plant Biotechnology
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