Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Short Communication
Identification of the glutathione S-transferase gene responsible for flower color intensity in carnations
Nobuhiro SasakiYuzo NishizakiYasuhiro UchidaEigo WakamatsuNaoyuki UmemotoMasaki MomoseMasachika OkamuraHiroyuki YoshidaMasaatsu YamaguchiMasayoshi NakayamaYoshihiro OzekiYoshio Itoh
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2012 Volume 29 Issue 3 Pages 223-227


Two cDNAs with homology to glutathione S-transferase (GST) were isolated from the carnation (Dianthus caryophyllus); these cDNAs are termed here DcGSTF1 and DcGSTF2. Phylogenetic analysis suggested that both DcGSTF1 and DcGSTF2 belonged to the Phi class of GSTs. DcGSTF2 showed high levels of transcription at late stages of petal development when anthocyanin biosynthesis is most active. Sequencing of DcGSTF2 indicated that it consisted of three exons and two introns. A truncated DcGSTF2 gene, resulting from the insertion of a CACTA-type transposable element, was found in the genome of a mutable flower line bearing deep pink sectors on pale pink petals. A full length DcGSTF2 gene driven by a continuous expression promoter was introduced into the epidermal cells of carnations with pale pink petals. The transformed cells were deep pink. These results suggest that the DcGSTF2 gene is responsible for flower color intensity in carnations.

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© 2012 by Japanese Society for Plant Cell and Molecular Biology
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