Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Original Paper
Overexpression of Q/q-related homoeoalleles of hexaploid wheat reveals distinct recovery of flower transformation in the apetala2 mutant of Arabidopsis
Parisa AbdollahiYoko KamiyaKanako KawauraYasunari Ogihara
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JOURNAL FREE ACCESS

2012 Volume 29 Issue 3 Pages 245-252

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Abstract

The Q gene has played substantial roles in wheat domestication. As it pleiotropically governs domestication-related traits, such as free threshing, glume shape and tenacity, rachis fragility, spike length, plant height, and flowering time, wheat is cultivated in widespread adaptation. The Q gene located on the A genome encodes the APETALA2-like transcription factor WAP2AQ. The allelic mutation from q (WAP2Aq) to Q took place in the polyploidy wheats, and the B and D genomes of bread (hexaploid) wheat conferred its homoeoalleles (WAP2B and WAP2D, respectively). Although WAP2Aq and WAP2D revealed allelic phenotypes against WAP2AQ, their functions remain to be clarified. We overexpressed full-length cDNAs of WAP2AQ, WAP2Aq, and WAP2D in the ap2 mutant line of Arabidopsis. WAP2AQ fully recovered their flower organs similar to the wild type, WAP2D showed less recovery, and WAP2Aq rescued the least mutant flower phenotype. Use of a yeast two-hybrid system showed that WAP2AQ formed the most homodimers, WAP2Aq formed the next highest, and WAP2D formed the least. The sequence comparisons between the three transcription factors and with AP2 of Arabidopsis revealed that WAP2Aq confers two single protein substitutions, I329-to-V substitution and K108-to-E in the nuclear translocation signal, WAP2D harbors SNPs of I329-to-L similar to Arabidopsis, and other 6 substitutions. These data support the idea that a critical point mutation at the functional domain and structure alteration(s) resulting from sequence diversifications caused functional differences in the genes. Mutant lines of Arabidopsis can become a powerful tool for analyzing foreign gene functions as in the case of wheat.

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© 2012 by Japanese Society for Plant Biotechnology
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