Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Short Communication
Somatic embryogenesis in leaf tissue culture of Soapberry (Sapindus mukorossi Gaertn.)
Hyun-Tae KimByeung-Hoon YangYoung Goo ParkJang R. Liu
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2012 Volume 29 Issue 3 Pages 311-314


Leaf explants formed embryogenic calluses at a frequency of 53.9% when cultured on B5 media supplemented with 0.1 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.01 mg l-1 6-benzyladenine (BA) for 6 weeks. Upon transfer onto media with 5 mg l-1 abscisic acid, embryogenic calluses yielded somatic embryos at 73%. Somatic embryos developed into plantlets on media without plant growth regulators at 90%. Embryogenic calluses proliferated and maintained embryogenic capacity when subcultured on media with 0.1 mg l-1 2,4-D and 0.01 mg l-1 BA at 4-week intervals. This culture system is an effective means for clonal propagation and genetic manipulation of soapberry because it ensures taproot development required for tree stability.

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© 2012 by Japanese Society for Plant Biotechnology
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