Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
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Production of transgenic rice plants expressing Dioscorea batatas tuber lectin 1 to confer resistance against brown planthopper
Shoichiro YoshimuraMasaaki KomatsuKoichiro KakuMasatoshi HoriTomohisa OgawaKoji MuramotoTomohiko KazamaYukihiro ItoKinya Toriyama
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2012 Volume 29 Issue 5 Pages 501-504

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Abstract

Dioscorea batatas tuber lectin 1 (DB1) is a storage protein isolated from yam tuber and has been shown to be a mannose-binding lectin. Here we produced transgenic rice plants expressing cDNA of DB1 under the control of phloem-specific promoter of rice sucrose synthase-1 gene. DB1 accumulated at a level of 0.07% of total soluble protein. We then evaluated its efficacy for brown planthopper (BPH). After releasing the first instar of BPH on the transgenic rice plants, the number of survived BPH adults was reduced up to 30% compared to that of wild-type rice. The number of the next generation BPH was suppressed to 22% on average in the seven most-resistant plants compared to that of wild-type rice plants when female adult BPH was inoculated. These results demonstrated that DB1 is effective to confer BPH resistance in terms of decreased survival and fecundity.

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© 2012 by Japanese Society for Plant Cell and Molecular Biology
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