Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
Short Communications
An efficient plant regeneration through embryogenic callus formation and direct somatic embryogenesis via immature embryo culture in Ipomoea purpurea and I. tricolor
Hiroki IshikuroEiji NitasakaMotoyasu Otani
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2014 Volume 31 Issue 2 Pages 179-183


We established an efficient plant regeneration system to form embryogenic calli in common morning glory (Ipomoea purpurea) and blue morning glory (I. tricolor). Immature embryos of both morning glories cultured on media containing 1 mg l−1 4-fluorophenoxyacetic acid (4FA) and 6% sucrose formed many embryogenic calli. The frequency of embryogenic callus formation was highest in I. purpurea strain Q74 (42.5%) and in I. tricolor cultivar ‘Flying Saucers’ (36.7%). Embryogenic callus formation differed with the genotypes in both morning glories. Numerous somatic embryos were formed from the embryogenic calli when the calli were transferred onto plant growth regulator-free medium.

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© 2014 by Japanese Society for Plant Cell and Molecular Biology
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