Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Identification of a xylem sap germin-like protein and its expression under short-day and non-freezing low-temperature conditions in poplar root
Tsutomu AoharaHiroaki MizunoDaiki KiyomichiYuta AbeKaoru MatsukiKeiko SagawaHitoshi MoriHiroaki IwaiJun FurukawaShinobu Satoh
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2016 Volume 33 Issue 2 Pages 123-127


In the shoots of photoperiod-sensitive deciduous trees, including poplar, short-day and non-freezing low-temperature conditions induce bud dormancy and its break, respectively, and these conditions also induce shoot cold acclimation. In a previous study, levels of organic and inorganic components, including proteins, increased in the xylem sap of Populus nigra in winter, suggesting seasonal changes in root functions. Here, analysis of a major xylem sap protein (XSP24) of P. nigra in winter by mass spectrometry together with the whole genome sequence of P. trichocarpa and transcript abundance in roots under short-day conditions identified PtXSP24 to be a germin-like protein of the cupin superfamily, which was reported to be associated with various stresses and to have oxalate oxidase and/or superoxide dismutase activities in the cell wall. Expression of XSP24, which corresponds to PtXSP24 in P. maximowiczii, a potentially useful Japanese native poplar in the same phylogenetic clade as P. trichocarpa, was enhanced under short-day and non-freezing low-temperature conditions, as well as by application of abscisic acid. These results suggest that XSP24 is involved in tolerance to environmental stresses in autumn and early winter.

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© 2016 by Japanese Society for Plant Biotechnology
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