Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Original Paper
Embryogenic callus induction and Agrobacterium-mediated genetic transformation of ‘Shine Muscat’ grape
Ikuko Nakajima Masaki EndoTakashi HajiTakaya MoriguchiToshiya Yamamoto
ジャーナル フリー

2020 年 37 巻 2 号 p. 185-194


We established a method for embryogenic callus induction and highly efficient Agrobacterium-mediated genetic transformation of a table grape cultivar ‘Shine Muscat’ (Vitis labruscana). Embryogenic calli were induced using flower bud filaments from a dormant cane. Agrobacterium strain LBA4404 harboring the binary plasmid pBin19-sgfp, which contains the sgfp and nptII genes, was used to infect embryogenic calli. Infected calli were selected on 1/2 MS medium containing 5% maltose and 2% agar supplemented with 15 mg l−1 kanamycin. Efficiency of transformation of regenerated plants reached nearly 100% as determined by PCR and Southern blot analyses. The developed method will open a new avenue for genome editing of ‘Shine Muscat’ and contribute to the advancement of grape breeding.

© 2020 The Japanese Society for Plant Cell and Molecular Biology
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