1996 Volume 13 Issue 3 Pages 259-264
An efficient method for plant regeneration from suspension cultures of onion was studied. For callus induction, sterilized seeds were inoculated on solid MS medium supplemented with 50μM 4-FPA, 1μM 2iP, 1g/l casein hydrolysate and 0.1M sucrose. For suspension culture, callus was mechanically disaggregated and transferred into liquid MS medium with 10mM MES added, and placed on a rotary shaker. Suspension cultures were routinely subcultured. After pre-culture in liquid medium which increased sucrose concentration to 0.2M, two types of cell clumps developed, a deep yellow, small type, and a light yellow, large type. The large type showed higher regenerative ability than the small type. Frequent regeneration of plantlets occurred on solid MS medium supplemented with 1-5μM 2iP. Regenerated plantlets developed to whole plants.