1996 年 13 巻 3 号 p. 317-320
Calli were induced from tuber explants of Colocasia esculenta Schott cv. Dodare on a basal MS medium supplemented with BA, NAA, 30g/l sucrose and 8g/l agar for 130 days at 26°C in the dark. Protocorm-like calli were obtained by culturing whitish calli on a phytohormone-free MS medium for 30 days at 26°C with a 16-hour photoperiod, and plants were regenerated from them. An in vitro clonal propagation method was achieved by culturing regenerated young plants derived from whitish callus on a phytohormone-free MS medium at 26°C with a 16-hour photoperiod.