Plant Regeneration from Leaf Explants of Primula cuneifolia var. hakusanensis, “Hakusan-kozakura”

Abstract

Somatic embryos and adventitious shoots were initiated from leaf explants of Primula cuneifolia var. hakusanensis on the media containing various cytokinins. The best treatment for induction of somatic embryos was on the medium containing 1.0mg/l of TDZ or 5.0-10.0mg/l of zeatin. Kinetin had no effect on the regeneration from leaf explants. Somatic embryos and adventitious shoots grew to plantlets on LS-hormone-free medium. After acclimatization in the growth chamber, the regenerated plantlets flowered and set seeds.