Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
Transgene inactivation in Agrobacterium-mediated chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) transformants
Yasumasa TAKATSUMikio HAYASHIFumio SAKUMA
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2000 Volume 17 Issue 3 Pages 241-245

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Abstract

To compare transformation frequency and to investigate the developmental alterations in transgene expression, two cut-flower chrysanthemums ‘Yamabiko’ (spray-type) and ‘New Summer Yellow’ (standard-type) were transformed with three disarmed Agrobacterium tumefaciens strains C58C1, MP90 and LBA4404, all strains having the pBI121 plasmid. No marked difference was observed in transformation efficiency among cultivar/bacterial strain combinations. β-glucuronidase (GUS) activity levels in transformants were fairly low and varied among the different transgenic lines, ranging from 30 to 250pmol min-1 (mg protein)-1. GUS expression was not observed in the transgenic lines transformed with strain LBA4404. The alterations of GUS activity levels were examined for a long term, and it was observed that GUS activities reduced to zero level in most of transgenic lines 12 months after the inoculation of bacteria.

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© Japanese Society for Plant Cell and Molecular Biology
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