A new procedure for collecting seeds of eelgrass (Zostera marina) was developed to minimize the damage to an original eelgrass bed. An appropriate treatment by fresh water to control the sprouting time was introduced in the seedling production, which not only improves the germination rate but also reduces the total time for seedling production. These procedures were tested in a field. Sixty shoots of Z. marina raised in tank were transplanted to sea area and increased to 1700 shoots in 18 months. This newly developed technique will be of enormous help in the speedy restoration of eelgrass bed while preserving the genetic diversity of Z. marina.