Poor understanding in relation to their aggregation hampers prediction of the suspension of the estuarine sediment. In this study, in situ experiment of the re-suspension of the estuarine sediment was conducted in an urban estuary, the Tsurumi River, Japan. The experiment was consists of the forceful suspension of sediments by the water jet pump and measurement of the turbulence, OBS and particle diameter. As a result of the experiment, It is clarified that surface layer of the sediment is easy to suspension and their clay particles were aggregated over 40%. It is concluded that low concentration of SS in the surface mud and aggregation of the clay particles resulted to high erodability estuarine fine sediment.