Double-Tracer Radiography with Sulfur-35 and Phosphorus-32 Using Imaging Plates in Brassica napus L.

Abstract

We present double-tracer autoradiography with ³⁵S and ³²P using imaging plates and an aluminum plate to visualize the individual distribution of sulfur and phosphorus in Brassica napus L.. Because of the different attenuation efficiencies of β-rays emitted from ³⁵S and ³²P, the specific image of ³²P was taken when an aluminum plate was placed between the sample and the imaging plate. The specific image of ³⁵S was obtained by subtracting the ³²P image from the ³⁵S and ³²P image. It was observed that high amount of ³⁵S was distributed at the edge of the leaves and in root tips. The ³²P was distributed throughout the whole plant and it was found that the phosphorous amount was higher at the shoot apical meristems than those in leaves.