2022 Volume 71 Issue 3 Pages 179-184
Tracer experiments using radioisotope are powerful for the analysis of mineral uptake mechanisms in plant roots. In this study, we aimed to establish a quantitative method for measuring ion fluxes, i.e. net flux, influx, and efflux, which can be used to phenotyping of the mutants and natural variations. Consistency of obtained flux values for Mg2+ and Na+ was examined, and it was found that radioisotope absorption time of 20 s gave influx rate and an absorption time of more than 15 min gave net flux rate in rice roots. Furthermore, it was suggested that the longest possible radioisotope labeling time is necessary to increase the accuracy of the calculated efflux rate.