Online ISSN : 1884-4111
Print ISSN : 0033-8303
ISSN-L : 0033-8303
Development of the Practical Method for Ion Influx, Efflux, and Net Flux Measurement in Plant Roots
Natsuko I. Kobayashi Xiaoyu YangRen IwataHisashi SuzukiTomoko M. NakanishiKeitaro Tanoi
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2022 Volume 71 Issue 3 Pages 179-184


Tracer experiments using radioisotope are powerful for the analysis of mineral uptake mechanisms in plant roots. In this study, we aimed to establish a quantitative method for measuring ion fluxes, i.e. net flux, influx, and efflux, which can be used to phenotyping of the mutants and natural variations. Consistency of obtained flux values for Mg2+ and Na+ was examined, and it was found that radioisotope absorption time of 20 s gave influx rate and an absorption time of more than 15 min gave net flux rate in rice roots. Furthermore, it was suggested that the longest possible radioisotope labeling time is necessary to increase the accuracy of the calculated efflux rate.

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© Japan Radioisotope Association 2022. This is an open access article distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) License (
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