A Criterion on the Histologic Fixation for Radioautograph A Release of Radioactivity from the Liver Tissue Treated with ³⁵S-Sulfate

Abstract

An attempt was made to study possible relations between histologic fixatives and radioactivity maintained in tissue specimens. The specimens were obtained from the liver of rats treated in vivo with ³⁵S-sodium sulfate. Radioactivities released from or kept in tissues were measured after fixation with various agents. An assumption that fixatives prevent a release of radioactivity from tissue specimens has been evaluated throughout the present experiments as follows: As a single fixative fluid, non-aqueous fluid, such as ethanol, shows the highest inhibition of the release. Of the aqueous fluids, trichloroacetic acid and lead acetate show an inhibition of the release which is denied to formalin. In the case of mixed agents, when aqueous fluids are employed in combination with respective, indicated agents for radioactive substances, the release may be inhibited, as shown by an additional use of barium chloride or calcium chloride for fixing radioactivity of ³⁵S-sulfate in tissue.