Abstract
Blasticidin S, an antifungal substance, showed inhibitory effect on Chlorella pyrenoidosa Chick and Nicotiana tobacum L, , but no effect was observed at the concentration less than 1 ppm and static effect at the concentration less than 100 ppm.
The inhibition of 14C-amino acids incorporation into protein fraction occurred on the cell free system rather than the intact cell of chlorella, and the inhibition was specifically observed on the protein synthesis of 15, 000×g-ppt fraction containing chloroplasts and mitochondria.
The similar results were obtained by the studies of the cell free system of Nicotiana tobacum.