Metabolism of Chinoform (5-chloro-7-iodo-8-quinolinol) -¹⁴C in Dog

Abstract

Chinoform (5-chloro-7-iodo-8-quinolinol) -¹⁴C (302.4μCi/83 mg) packed in a capsule was given orally to a dog weighing 10.5 kg and urine and feces were collected for 5 days after dosing. The excretions of large portion of radioactivities in urine and feces were observed at the 1st day and 3rd day after dosing, respectively. The total radioactivity in both excreta in 5 days was about 70% of dose, and the urine was used for the study of metabolites.
The urine was extracted with a mixture of benzene and pyridine (9: 1), and organic layer and aqueous layer were obtained.
Intact chinoform and 5-chloro-8-quinolinol (a deiodinated product of chinoform) were identified in the organic layer by radiochromatography and isotope dilution method. From the water layer of urine, glucuronides of chinoform and the deiodinated product, and sulfate of chinoform were identified as the metabolites.