1975 Volume 24 Issue 7 Pages 479-485
Problems on the determination of serum vitamin B12 values using Phadebas B12 test kit were studied.
Various causes considered to influence the results were investigated.
There was no release of bound 57Co-B12 from Sephadex-intrinsic factor complex.
Standard curves which were expressed by percentages of each sample versus zero sample, were slightly different each other on their forms and inlines. Variations of percentage of zero samples showed the standard deviation of ±9.2% of mean value. Percentage of B/T in various concentration of B12 showed also one standard deviations of 8-9%. There were no inhibitory effects on the /resence of serum in the sample to binding ability of B12 to Sephadex-intrinsic factor complex.
It was more suitable to use 0.5 ml of extracted dilution of serum did not change the measured B12 values and standard curve was more accurate in lower side of B12 concentration than in higher side. The reproducibility and recovery of serum B12 values showed quite excellent results by this method.
Phadebas B12 test kit gave correct serum B12 values in general without difficult techniques. Then it was concluded that Phadebas B12 test kit was one of the most suitable method for the determination of serum B12 values in clinical samples.