Fundamental Studies on the Development of C-peptide Radioimmunoassay Kit

Abstract

We have studied the development of C-peptide radioimmunoassay kit which is usable in the pancreatic function test with satisfactory results. The C-peptide antiserum was prapared by immunizing rabbits with synthetic human connecting peptide. The antiserum revealed no cross reaction to any other C-peptides except human C-peptide, porcine insulin and gastrointestinal hormon, and showed high specificity to human C-peptide.
We adopted double antibody method in B, F separation, and chose 4°C, 48 hes. for 1st. incubation and 4°C, 24 hrs. for 2nd. incubation.
On this kit, the assay range was 0.5 ng/ml-30ng/ml, the recovery rate was 98.4%-107.8% in the recovery test, the coefficient of variance was 6.2% on the intra assay and was 7.6% on the inter assay.
We think this kit is sufficiently usable to assay the C-peptide in blood.