1977 Volume 26 Issue 3 Pages 169-174
Subcellular distribution of 169Yb and 111In was quantitatively determined to evaluate the role of lysosome in accumulation of 169Yb and 111In in malignant tumor tissue and liver. The following animals and transplanted tumors were used: rats implanted with Yoshida sarcoma and hepatoma AH109A; mice implanted with Ehrlich tumor. 169Yb-citrate and 111In-citrate were injected to the rats intravenously and to the mice intraperi-toneally. Ten minutes to 48 hours after the administration of 169Yb-citrate and 111In-citrate, the animals were sacrificed, and the tumor tissues and liver were excised. Subcellular fractionation of tumor tissues and livers were carried cut according to the method of Hogeboom and Schneider. 169Yb and 111In of each fraction was counted by a well type scintillation counter, and protein of each fraction was measured according to Lowry's method.
In Yoshida sarcoma and Ehrlich tumor, most of the radioactivity was localized in the supernatant fraction, and small amount of radioactivity was accumulated in the mitochondrial fraction (lysosome contains in this fraction) . But in the liver, most of the radioactivity was concentrated in the mitochondrial fraction and the radioactivity of this fraction was increased with the passage of time after administration. Twenty-four hours later, about 50% of total radioactivity was accumulated in this fraction. In the case of hepatoma AH109A, . radioactivity of mitochondrial fraction was increased. with time after administration, and about 30% of total radioactivity was concentrated in this fraction 24 hours after administration.
From these results it is concluded that lysosome does not play an important role in the tumor concentration of 169Yb and 111In and lysosome plays an important role in the liver, cancentration of 169Yb and 111In. In the case of hepatoma AH109A it is presumed that lysosome plays cosiderably important role in the tumor concentration of 169Yb and 111In, as hepatoma AH109A remains some nature of liver.
