1983 Volume 32 Issue 12 Pages 617-622
The technique of freezing whole body autoradiography using positron emitting nuclides has been developed in the present study.
Because of rapid decay of positron emitters, a frozen section to contact with a X-ray film must be prepared within a few hours. All the procedures to obtain autoradiograms of rats using 18F-2-deoxy-2-fluoro-D-glucose (18F-FDG) or 11C-glucose-fructose mixture (11C-glucose) were described in detail.
In the present technique, exposure must be performed at-20°C to keep a section frozen. Density of X-ray films after exposure at 20°C or-20°C was examined using various radioactive sections. The reduction of the density exposured at-20°C was observed. But the density exposured at-20°C was proportional to the radioactivity.
Freezing whole body autoradiography of rats bearing subcutaneous AH109A tumors was performed using 18F-FDG (half life of 109.7 min) and 11C-glucose (half life of 20 min) . Density of tumors and other organs on the autoradiogram was comparable with tissue distribution studies of 18F-FDG and 11C-glucose.
Freezing whole body autoradiography can be feasible for the analysis of tissue distribution on positron emitting radiopharmaceuticals.