免疫放射定量分析法によるHBs抗体の定量に関する基礎的検討
1989 年 38 巻 7 号 p. 319-322
詳細
1989 年 38 巻 7 号 p. 319-322
Quantification of HBs-antibody assay was carried out using a commercialised assay kit and standard solutions of HBs-antibody recognised as 1 St reference preparation of hepatitis B immunogloblin by WHO.
Standard curve of HBs-antibody was drawn with the function of 3D-spline and the correlation factor was obtained as r=0.999. Coefficient of intra-assay variance was 3.8% and that of inter-assay variance was 7.8%. Dilution tests showed satisfactory results in the range of 2-16 times. Correlation between value of cut-off indices and concentration of HBs-antibody was obtained as the formula of y=2.599 x -3.894 (r=0.992) and 2.1 of cut-off index corresponded to about 5 mIU/ml of HBs-antibody concentration.
