1993 Volume 42 Issue 9 Pages 511-516
Quantitative analysis of aminopyrine (AM) and its deuterated analogues (AM -d6and AM-d9) were carried out by gas chromatography. The separation of protio- and deutero forms of AM was achieved by gas chromatography using a fused-silica capillary column (25 m) . The resolution coefficients between AM and deuterated AM were 1.05 for AM-d6and 1.52 for AM d9, respectively.
The present isotopic fractionation procedure was applied to the isotope dilution analysis of AM By the measurement of the samples prepared by the addition of known amount of AM and AM d9to the control blood of rat, a linear relationship between peak area ratio and added amount ratio was observed. The correlation coefficients obtained by regression analysis was 0.9999.
The present method was applied to determine the blood level of AM in rat after i.v. injection of AM The kinetic parameters of AM, Vd, kel and total body clearance, were estimated from the time course of blood concentration of AM The pretreatment with carbon tetrachloride or Phenobarbital led to a significant change in the kel and the clearance of AM The present results indicate that this isotopic fractionation would be a suitable method for evaluation of hepatic function.
