Abstract
The present paper is concerned with a method of thin layer starch gel electrophoresis modifying the method of Baur. Characteristics and advantages of this method are the following: (1) proteins are clearly separable by means of a relatively simple technique, and (2) many samples are at the same time analyzed only for two hours.
Two square glass plates (size 20×20 cm) are prepared as a pair, one of which is fraund with vinyl tape of 2 mm thickness for the square and another is used as the cover.
Gel preparation buffer (pH 8.6) is 0.9 M tris (hydroxymethyl) aminomethane, 0.02 M EDTA and 0.5 M boric acid as a stock solution. This stock solution is diluted 15 times with water before use. The electrode buffer (pH 8.6) is 0.18 M boric acid and 0.08 M sodium metaborate.
Ten gm of hydrolysed starch (Connaught Laboratories) is mixed with 60 ml of gel preparation buffer.
Concentrations of hemolysate, serum and urine are approximately 10 gm/dl, 7 gm/dl, and 2—6 gm/dl, respectively.
Electrophoresis was performed 250 volts/20 cm for 2 hours and stained with amido black 10 B. Using electrophoresis of this system for screening of abnormal hemoglobins, we have detected 22 abnormal hemoglobins and 6 thalassemias among 60,000 blood specimens in Western Japan.
