抄録
Anti-human prothrombin was obtained from rabbits immunized with purified human prothrombin which was prepared from citrated human plasma by DeVilee's method. There was statistically significant correlation between the immunologicaly assayed-plasma prothrombin level and result of conventional coagulation tests representing the factor II concentration. Immunologicaly measurement for plasma prothrombin level will, therefore, be useful as one of the coagulation tests.
Plasma prothrombin content of patients with various liver diseases were measured by the immunological methods. Abnormal plasma prothrombin level was detected clearly in the majority of them, and it was especially marked in patients with cirrhosis of the liver. Plasma prothrombin levels by the immunological assay were 12.5±2.4mg/dl (mean+SD) in healthy people, 6.5±2.5mg/dl in acute hepatitis (active stage), 10.6±3.7mg/dl in acute hepatitis (covalescent stage), 9.06±2.2mg/dl in chronic inactive hepatitis, 8.4±2.4mg/dl in chronic active hepatitis and 6.0±2.1mg/dl in liver cirrhosis. In acute hepatitis, particularly in fulminant hepatitis the plasma prothrombin levels mirrored clinical course more sensitively and precisely than did the serum albumin levels. The plasma prothrombin immunologically assayed will be a useful index for the impaired liver functions in hepatobiliary diseases.
