Abstract
Terminal transferase (TdT)-positive cells were surveyed in leukemia cases by indirect immunoperoxidase method. The presence or the absence of TdT and the ratio of TdT-positive cells assessed by immunoperoxidase method were consistent with those assessed by immunofluorescence of TdT. Peroxidase-positive substance was detected as the small granules in the nucleus or both in the nucleus and cytoplasm of the blasts from TdT-positive leukemia cases. Replacement of rabbit anti-TdT serum with PBS or with normal rabbit IgG gave negative staining in the slides from TdT-positive leukemia cases.
Immunoperoxidase method is an easy and excellent way to survey TdT in leukemia cases.
