Abstract
A microplate enzyme linked immunosorbent assay (ELISA) has been developed for quantitation of small amount of red blood cell-bound IgG from hematologically normal subjects. A double antibody sandwich method used in this study was highly sensitive and reproducible. The number of IgG molecules bound to a normal red cell was ranged from 15 to 104 with an average of 55 (N=30). Because contamination of buffy coat provides a high titer (false positive result), it is important to remove buffy coat as completely as possible by repeated washing of red cells.
