1987 Volume 1 Issue 1 Pages 31-42
The morphology of Corynebacterium glutamicum ATCC 13032 due to biotin in a medium was studied by electron microscopy. The thin- sectioned cell envelope of plasmolysed normal cells revealed three electron-dense layers within the cell wall. Freeze-cleavage occurred at different levels of the cell wall, predominantly between the middle and outermost layers. The main convex faces consisted of closely packed particles and main concave faces consisted of roughly studded small particle areas.
Distinct alterations of the cell wall caused by biotin-deficiency were the formations of blebs and the rearrangements of particles on the main concave faces. The blebs had similar striated structures composed of parallel lines on both fracture faces.