抄録
Main studies on Streptomyces, especially S. griseus, during about thirty years of the author’s career are outlined. 1. Studies on streptomycin biosynthesis using resting mycelium-suspension culture of S. griseus revealed the following; 1) Streptomycin 6-phosphate is the final precursor, which is converted to streptomycin by a specific alkaline phosphatase, 2) Streptomycin 6- phosphotransferase contributes to self-protection from a toxic effect of streptomycin, and 3) Suppression of the phospholipid cycle of cell wall synthesis results in the accumulation of glucosamine 6-phosphate in the resting mycelium and the enhancement of streptomycin production 2. Genetic studies provided the following; 1) S. griseus has two genes contributing to self-protection against streptomycin, 2) Pleiotropic mutants were obtained at a high frequency by incubating mycelium under stress condition for growth, and 3) Physical macrorestriction map of S. griseus 2247 strain was constructed.
