Construction of Escherichia coli-Streptomyces Shuttle Vectors Transducible in Streptomyces

Abstract

Shuttle vectors, pR4C10 and pR4C11, composed of Streptomyces cosmid and Escherichia coli plasmid were constructed. With both plasmids, Streptomyces lividans and Escherichia coli K12 strains were transformed at high frequencies, comparable with the original plasmids. A vector, pR4Cll, was shown to be encapsidated into particles of actinophage R4 in vivo, and transferred to Streptomyces lividans.