Application of mass spectrometry for the life sciences, especially for the proteome analysis, has attracted considerable attention as it shows high potential for the complete of comprehensive analysis of protein expressions. Although the technology progress of the mass spectrometry is outstandingly fast, there are a few difficulties to solve in the peripheral areas. In this report, we focus on the sample preparation in the up-stream of mass spectrometry and the data analysis method at the down-stream of mass spectrometry, and introduce/discuss the current and new technologies.
