マイクロチップ電気泳動法と試料濃縮

抄録

Sample solutions are often introduced to microchip electrophoretic systems by electrokinetic injection, which often causes selective injection of the components with higher electrophoretic mobility, and impairs the reliability of quantitative analysis. One promising alternative to overcome this limitation is on-line sample trapping enabling the concentration of all analytes in sample reservoir to the separation channel. Here we describe the methodology of concentration techniques of field amplified stacking, micellar sweeping, isotachophoresis, perm-selectivity, and solid phase extraction mainly concerned on microchip electrophoresis. Then we also introduce our recent research on highly effective preconcentration of anionic samples using sulfonate-type polyacrylamide gel fabricated by in-situ photopolymerization.