生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
特集:最新のキャピラリー電気泳動とチップ電気泳動
抗体医薬品開発及び品質管理におけるキャピラリー電気泳動法の応用
新井 悦郎
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ジャーナル フリー

2008 年 52 巻 3 号 p. 139-144

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Recently the development and the practical application of monoclonal antibodies for clinical use are rapidly expanding in pharmaceutical business trend. Because of the heterogeneities of antibodies as glycoproteins, a new approach to test the products is required. Capillary electrophoresis (CE) is one of the important candidates that meet this demand.
CE separation is based on the various modes involving capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF). All of them are used for the analysis and test of monoclonal antibodies for clinical use. Especially with CGE, a non-glycosylated heavy chain is separated from a glycosylated one and detected. CIEF is an important technology to analyze the heterogeneity of antibodies mainly based on the N-linked oligosaccharide composition.
N-linked oligosaccharide structure is also important as it effects on the bio-activity of the antibodies like antibody-dependent cellular cytotoxity. The fluorescence and charge insertion into oligosaccharides by chemical labeling is a valuable strategy to make CE useful for the analysis of them which released by enzyme digestion of antibodies.
CZE is also useful not only for peptide mapping but also as the base of the CE-mass spectrometry approach to analyze the structure of peptides and N-linked oligosaccharide chains.
These applications are discussed with examples.

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© 2008 日本電気泳動学会
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