1) With a view to making clear the difference between immunisations with living and dead bacteria, we studied experimental typhoid fever of rabbits, especially on agglutination, onset-inhibiting power and alteration of serum protein in electrophoretic analysis.
2) Dividing rabbits into two groups we vaccinated the first group with weak living S. cholerae suis (R type P 32 strain) and the second group with dead S-type bacteria.
After vaccinating them, we inoculated both groups with the strong “Hokkaido-Strain”.
The second group died though they showed strong agglutination. Those in the first group all remained alive, though agglutination was negative.
3) Total serum, protein increased in all cases except one. γ-Globulin showed the most remakable increase, namely two or three fold. We were not able to recognize quantitative difference in γ-globulin between these two groups.
However, the shape of the peak showed a difference. In the first group the shape of the peak of γ-globulin is larger on the side of larger mobility, than on the side of a smaller mobility.
The conditions in those of the second group are completely reverse.
4) It was supposed that there were some relations among the shape of the γ-globulin-peak, agglutination and general immunisation.