濃度勾配ポリアクリルアミドゲル電気泳動法によるHDL亜分画の測定

抄録

We established a simple method for measuring HDL subfraction cholesterols by gradient polyacrylamide gel electrophoresis (gradient PAGE). Prestained human serum was separated into two major bands identical with HDL₂ and HDL₃ by the gradient PAGE. HDL-cholesterol (HDL-C) obtained by precipitation method was divided according to HDL₂/HDL₃ ratio determined by densitometry of the electrophoretic pattern. HDL subfraction cholesterols determined by this method was well correlated with those measured by zonal ultracentrifugation (HDL₂-C: r=0.98, HDL₃-C: r=0.94, n=11). Mean serum HDL₂-C level was significantly higher in normal females than in normal males (p<0.01). HDL₃-C levels were significantly decreased in liver diseases, especially in cirrhotics. This simple electrophoretic method for quantification of HDL₂ and HDL₃ is suitable for clinical application in various pathological states.