水平式SDS電気泳動によるVLDL中のアポリポ蛋白の分析に関する研究
1983 年 27 巻 6 号 p. 331-336
詳細
1983 年 27 巻 6 号 p. 331-336
A horizontal sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was applied to the study of apolipoprotein components of the human serum very low density lipoprotein (VLDL). By this method, tetramethylurea(TMU)-soluble apolipoproteins could be separated into four major fractions, called apolipoprotein CI (apo CI), CII, CIII and E from anode to cathode.
The distribution of the major soluble apolipoproteins of VLDL in 9 healthy adults as percentage (mean±SD) of the total soluble apolipoprotein was: apo CI, (7.5±3.8): apo CII, (19.8±6.3): apo CIII, (50.0±6.7) and apo E, (22.7±5.4).
The composition of apolipoproteins of VLDL in 9 patients with hypertriglyceridemia was characterized by a decrease of apo CII (13.5±3.1) and an increase of apo CI (10.4±2.3). The results suggest that the variation of apo CII and CI content carried on VLDL may be critical for their interaction with lipoprotein lipase.
