Abstract
A simple method for fractionation and detection of serum sialoproteins is described. The method is based on the combination of cellulose acetate membrane electrophoresis and sialic acid staining using enzyme reactions of neuraminidase NANA-aldolase, pyruvate oxidase and peroxidase.
The results obtained are as follows:
1) Total serum sialic acid in 52 healthy Japanese ranged from 53 to 73mg/dl, and serum sialoproteins were separated into fractions with α1-, α2- and β-globulin mobilities, the relative contents (mean±2SD) being 39±14, 40±16 and 20±14%, respectively.
2) Another band of sialoprotein was found in position of the electrophoretic mobility of M-protein in some cases of M-proteinemia (IgG, IgA, IgM and B-J).
The method may be useful for screening test of qualitative analysis of serum sialoproteins.
