SEIBUTSU BUTSURI KAGAKU
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
Purification and properties of α1- and α2-macroglobulins in the dog
Kazuko SakaguchiFumiaki AkahoriKimio HamadaMiki KishiToshio MasaokaMitsuyuki ShiraiRyuko KohzakiAtsushi SawadaKen-ichi KohzakiSadao Kobayashi
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1992 Volume 36 Issue 2 Pages 139-143

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Abstract
1) The ultrafiltration of serum collected from dogs by a filtration film with a pore size of 300, 000, Blue Sepharose CL-6B affinity chromatography and Ultrogel AcA 22gel filtration chromatography eluted a third protein fraction in addition to α1-macroglobulin (α1-M) and α2-macrogrobulin (α2-M). The subsequent use of preparative agarose gel isoelectric focusing with a cover gel facilitated the extraction of pure α1-M and α2-M protein fractions.2) Analytical agarose gel isoelectric focusing and Ferguson plot by agarose gel electrophoresis revealed the following: (1) dog α1-M had an isoelectric point (pI) of 5.4 and a molecular weight of 724, 000; and (2) dog α2-M had a pI of 5.7 and a molecular weight of 758, 000.
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© by Japanese Electrophoresis Society
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