Purification and properties of α₁- and α₂-macroglobulins in the dog

Abstract

1) The ultrafiltration of serum collected from dogs by a filtration film with a pore size of 300, 000, Blue Sepharose CL-6B affinity chromatography and Ultrogel AcA 22gel filtration chromatography eluted a third protein fraction in addition to α₁-macroglobulin (α₁-M) and α₂-macrogrobulin (α₂-M). The subsequent use of preparative agarose gel isoelectric focusing with a cover gel facilitated the extraction of pure α₁-M and α₂-M protein fractions.2) Analytical agarose gel isoelectric focusing and Ferguson plot by agarose gel electrophoresis revealed the following: (1) dog α₁-M had an isoelectric point (pI) of 5.4 and a molecular weight of 724, 000; and (2) dog α₂-M had a pI of 5.7 and a molecular weight of 758, 000.