生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
濾紙電気泳動法の測定法の吟味,特にピペット法と紙片法との比較
林 喜男
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ジャーナル フリー

1958 年 5 巻 2 号 p. 52-55

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In the technique of Paper-electrophoresis, the size of the serum sample is an important factor, because a linear relation between protein concentration and densitometer reading depends upon it considerably.
While the original technique of applying the serum to an electrophoresis paper by a micropipet requires a skill acquired only by repeated practice, the use of an applicator strip which was used in this experiment, makes it easy to apply a constant amount of the serum sample. Furthermore, the above mentioned technique presents a well-separated pattern of serum protein, the separation of α1- and α2-globulin fractions being especially distinct.
The standard errors of the fractions are smaller compared with those by the micropipet method.

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© 日本電気泳動学会
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