In the technique of Paper-electrophoresis, the size of the serum sample is an important factor, because a linear relation between protein concentration and densitometer reading depends upon it considerably.
While the original technique of applying the serum to an electrophoresis paper by a micropipet requires a skill acquired only by repeated practice, the use of an applicator strip which was used in this experiment, makes it easy to apply a constant amount of the serum sample. Furthermore, the above mentioned technique presents a well-separated pattern of serum protein, the separation of α1- and α2-globulin fractions being especially distinct.
The standard errors of the fractions are smaller compared with those by the micropipet method.