Solvent Extraction Research and Development, Japan
Online ISSN : 2188-4765
Print ISSN : 1341-7215
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Enhanced Productivity of Paclitaxel and Related Taxanes in Plant Cell Culture Including Aliphatic Ionic Liquids
Shinjiro YAMAMOTOYuka SONODATakato KATAOKAShuhei HAYASHIHitoshi MIYASAKA
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2018 Volume 25 Issue 2 Pages 125-130

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Abstract

We report here on hydrophobic ionic liquids (ILs) which enhance the productivity of paclitaxel and the related taxanes of 10-deacetyl baccatin III, baccatin III and cepharomannine with in situ extraction from an aqueous medium in the suspension cell culture of Taxus cuspidata in the IL-medium two phase culture system. Two aliphatic ILs of N-methyl-n-propylpiperidinium bis(trifluoromethane-sulfonyl)imide (PP13-TFSI) and N-methyl-n-butylpyrrolidinium bis(trifluoromethanesulfonyl)imide (P14-TFSI) were used. It was found that the two ILs had no cytotoxicity and enhanced productivity of paclitaxel and the related taxanes in the cell culture. In particular, the more hydrophobic P14-TFSI, which enhanced the productivity of paclitaxel and the total taxanes by a factor of more than 2 compared to PP13-TFSI, could be an effective extractant.

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© 2018 Japan Association of Solvent Extraction
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