Effect of Methyl Jasmonate on Production of Paclitaxel and Related Taxanes in a Hydrophobic Ionic Liquid-Medium Two Phase Culture System

Abstract

We report here a hydrophobic ionic liquid (IL), 1-butyl-1-methylpyrrolidinium bis(trifluoromethanesulfonyl)imide (P14-TFSI), that contributed to increasing the amount of production of paclitaxel and the related taxanes of 10-deacetyl baccatin III, baccatin III and cepharomannine with in situ extraction from an aqueous medium in the IL-medium two phase culture system. Addition of an elicitor, methyl jasmonate (MJ) to the cell culture system enhanced the amount of production of the taxanes in the culture including 2.5 % IL. The total amount of the taxanes in the culture including 10 µM MJ and 2.5 vol% IL was more than 200 times greater than those in the control culture in the absence of IL and MJ, and more than two times greater than those in the culture including 2.5 vol% IL alone.