LipopolysaccharideおよびEndothelinがヒト歯髄由来細胞に及ぼす影響

抄録

Lipopolysaccharide (LPS) from gram-negative bacteria has various activities and is a common inflammatory factor in endodontic-periodontal lesions. Also, endothelin is detected in both normal and inflammatory pulp tissues, but it has still not been shown howendothelin is concerned with inflammation. The differences between the influences of LPS and endothelin on pulp tissues have also not yet been examined. The aim of this study was to reveal how LPS and endothelin influence human dental pulp cells (HDPC). We compared the biological activities between LPS and endothelin using cell proliferation, alkaline phosphatase (ALP) activity and the gene expressions of interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and heat shock protein (HSP), which is a stress protein. The cell proliferation of HDPC in endothelin was significantly higher than that in the control. However, cell proliferation of HDPC in LPS tended to increase. The Alp activity of HDPC was suppressed in endothelin, but the suppression of ALP activity in LPS was greater than that of ALP activity in endothelin. The mRNA expressions of HSP60 and HSP70 were not different between the control, LPS and endothelin. While the mRNA expression of IL-6 was induced by endothelin, the mRNA expression of IL-6 was more induced by LPS than by endothelin. Also, the mRNA expressions of HSP27 and COX-2 were induced in the control and endothelin. However, the mRNA expressions of HSP27 and COX-2 were more induced in LPS than that in the control and endothelin. In conclusion, it has been shown that the biological activities between LPS and endothelin are different, and that LPS and endothelin do not influence the cytodifferentiation of HDPC.