セイヨウワサビ毛状根からの不定芽分化におよぼす糖質および浸透圧の影響

抄録

Horseradish (Armoracia rusticana) hairy roots were established by transformation with the Ri plasmid T-DNA in Agrobacterium rhizogenes 15834. To elucidate the effects of sugar and osmotic pressure on the differentiation of adventitious shoots in horseradish hairy roots, the hairy-root clone AR450 was cultured in phytohormone-free LS medium containing sucrose, glucose,and fructose under light conditions. Adventitious shoots were formed in the presence of 1.5% (W/V) glucose or 1.5% (W/V) fructose as well as in the presence of 3% (W/V) sucrose. High osmotic pressure inhibited the formation of adventitious shoots in horseradish hairy roots. Adventitious shoots were also formed effectively in liquid LS medium without phytohormones under light conditions.