2000 Volume 41 Issue 3 Pages 206-211
A simple and rapid method for the determination of ellagic acid in fresh and processed fruits by HPLC with photodiode array detection has been developed. The sample was refluxed with methanol, and the extract was refined using a solid phase cartridge before HPLC. The recoveries from strawberries, pineapples and raspberries spiked with ellagic acid (25 μg/g and 50 μg/g) were 90.1∼98.3% (S.D. 0.9∼2.5%), and the limits of quantification were 0.05 μg/g.
We then analyzed ellagic acid in 41 kinds of fresh fruits and 11 kinds of processed fruits by this method. Ellagic acid was found in several berries [blackberry, strawberry, raspberry and bayberry], fueijoa, pineapple, pomegranate, and jams made from raspberry and strawberry. This is the first detection of ellagic acid in bayberry, fueijoa and pineapple.